recO [2020-03-03 11:56:09]

mediator of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] binding to ssDNA, required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair centers, required for efficient survival and replication restart after replication-transcription conflicts

locus: BSU_25280

pI: 8.21

mw: 29.20 kDa

protein length: 255 aa

gene length: 768 bp

function: DNA repair/ recombination

product: mediator of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] binding to ssDNA

essential: no

synonyms: yqxN,yqfI

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

Gene

Coordinates: 2,608,946 2,609,713

Phenotypes of a mutant

• drastically reduced survival of mature dormant spores after exposure to ultrahigh vacuum desiccation and ionizing radiation that induce single strand (ss) DNA nicks and double-strand breaks (DSBs) [Pubmed|24285298]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]

The protein

Catalyzed reaction/ biological activity

• provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] access to ssDNA during chromosomal transformation (together with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA]) [Pubmed|22373918]
• catalyzes annealing of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] or [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]/[protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation [Pubmed|22373918]
• required for the formation of [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] DNA repair centers (together with [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]) [Pubmed|24891441]
• anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
• [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]

Protein family

• recO family (single member, according to UniProt)

Structure

• [PDB|2V1C] (the [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]-[protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO] complex from ''Deinococcus radiodurans'', [protein|BEBAF1EB53EBB3E7558BB5FE73C418B50000FA29|RecR]: 52% identity, 78% similarity, [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|RecO]: 30%/ 58%) [Pubmed|17581636]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)
• localizes to the DNA entry pole during transformation [Pubmed|22373918]

Expression and Regulation

Operons

• genes: [gene|0E9E9615CE056BEA2B65C494D946EB45F42A3064|cdd]-[gene|CB68B3393CA7862C45C624AF7B1ADD188243A01F|era]-[gene|33FBC31E8C24177137B0BDD8B46C1AFFD7F7B038|yqzL]-[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|6330116], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|6330116], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

Biological materials

Mutant

• MGNA-C494 (yqxN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2492 NBRP B. subtilis, Japan]
• 1A892 ( ''recO''::''cat''), [Pubmed|10323239], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A892&Search=1A892 BGSC]
• BKE25280 ( ''recO''::''ermC''), (available at the [http://www.bgsc.org/ BGSC] and in [SW|Fabian Commichau]'s lab) [pubmed|28189581]
• BP738 ( ''recO''::''tet''), (available in [SW|Fabian Commichau]'s lab)
• BP774 ( ''recO''::''ermC''), (available in [SW|Fabian Commichau]'s lab)
• BKE25280 ([gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT
• BKK25280 ([gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCCGCACCTTCCTCAA, downstream forward: _UP4_TGACATTTGGTCCATCTTTT

References

Reviews

• 22933559

Original publications

• 24891441,18599486,19730681,20581116,22373918,17581636,21170359,24285298,24285298,25939832,26001966,15186413,10323239,26786319,23779106,30401797,31876108